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Osmosis in roots
Chemistry

Osmosis in roots

Osmosis moves water through roots, the concentration gradient across root membranes that lets a plant take up the nutrients dissolved in soil water.

Water, cells and plants
osmosis; root pressure; plant transport

The science behind this experiment

Osmosis is the movement of solvent molecules through a semi-permeable membrane due to a gradient of concentration between two solutions. This phenomenon is responsible for the absorption of nutritive substances through roots.

Equipment used

  • Beaker 500 ml — HEA-003
  • Cork borer — PLA-023
  • Dropping pipette with teats — PLA-003+PLA-019
  • Glass delivery tube — PLA-006
  • Carrot
  • Toothpicks

Chemicals and reagents

  • Concentrated sucrose solution
  • Methyl blue dye — R:20/21/22

Questions for students

  • Report your observations, trying to explain why the liquid level rises.

What to expect

After an hour the level of the liquid rises due to osmotic pressure. The concentration of sucrose is higher in the carrot and water therefore will tend to enter. The absorption of water stops when the weight of the liquid column balances the osmotic pressure.

Disposal

Drain the carrot into the acids and bases waste container.

Run this experiment in your classroom

This experiment is part of ATP Volta — a self-contained mobile science lab that turns an ordinary classroom into a working STEM laboratory, with over 200 experiments in physics, chemistry, biology, robotics and engineering. Gali, the AI tutor built into ATP Connect, guides students through each step and answers their questions at the bench.

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