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Chromatography of chlorophyll
Chimica

Chromatography of chlorophyll

To introduce a common organic extraction technique.

Acqua, cellule e piante
chromatography; chlorophyll; pigments; extraction

Cosa imparano gli studenti

La scienza alla base di questo esperimento

Chromatography is a technique used to purify and extract organic and inorganic substances from mixtures. It is based on the different speed of migration of various chemical compounds carried by a solvent (liquid phase) on a substrate (solid phase).

Attrezzatura utilizzata

  • Iron stand — 5404.52
  • Right angle clamp — 5401.16
  • Universal clamp — 5416.20
  • Mortar with pestle — 1951.05
  • Beaker 100 ml — 1118.20
  • Watch glass 80 mm — 1489.15
  • Conical flask 250 ml — 1322.20
  • Dropping pipette with teat — 1441.50+5448.00
  • Glass funnel — 1343.15
  • Filter paper square — 8349.20
  • Filter paper round — 8340.08
  • Spatola — 5460.34
  • Paper clip
  • Cotton thread
  • Adhesive tape

Sostanze chimiche e reagenti

  • Acetone — R:11-36-66-67 S:9-16-26
  • Ethanol — R:11 S:7-16
  • Diethyl ether — R:12-19-22-66-67 S:9-16-29-33
  • Leaves

Note sulla sicurezza

  • Work in a fume cupboard or outdoors.

Domande per gli studenti

  • Observe. What do the bands consist of?
  • Explain briefly the chromatography principles.

Cosa aspettarsi

<p>A series of bands is observed on the paper showing also different colours that indicate the presence of different.</p><p>Chromatography is based on the different speed of migration of the components due to their molecular interaction with solvents (mobile phase) and substrates (stationary phase).</p><p>As the paper absorbs the solvent this carries the pigments which in their turn interact with the paper moving with different speeds.</p>

Smaltimento

Dispose of the filter paper and the solid mass into the solids waste container. Dispose of the solvents into the flammables waste container.

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Questo esperimento fa parte dell’ATP “ Mobile Lab ” — un laboratorio autonomo che trasforma una normale aula in un vero e proprio laboratorio scientifico, con oltre 200 esperimenti di fisica, chimica, biologia, robotica e ingegneria. “ Gali ”, il tutor basato sull’intelligenza artificiale integrato in ATP Connect, guida gli studenti in ogni fase e risponde alle loro domande direttamente al banco di lavoro.

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