Chromatography of chlorophyll
To introduce a common organic extraction technique.
Lo que aprenden los alumnos
La base científica de este experimento
Chromatography is a technique used to purify and extract organic and inorganic substances from mixtures. It is based on the different speed of migration of various chemical compounds carried by a solvent (liquid phase) on a substrate (solid phase).
Equipo utilizado
- Iron stand — 5404.52
- Right angle clamp — 5401.16
- Universal clamp — 5416.20
- Mortar with pestle — 1951.05
- Beaker 100 ml — 1118.20
- Watch glass 80 mm — 1489.15
- Conical flask 250 ml — 1322.20
- Dropping pipette with teat — 1441.50+5448.00
- Glass funnel — 1343.15
- Filter paper square — 8349.20
- Filter paper round — 8340.08
- Spatula — 5460.34
- Paper clip
- Cotton thread
- Adhesive tape
Productos químicos y reactivos
- Acetone — R:11-36-66-67 S:9-16-26
- Ethanol — R:11 S:7-16
- Diethyl ether — R:12-19-22-66-67 S:9-16-29-33
- Leaves
Notas de seguridad
- Work in a fume cupboard or outdoors.
Preguntas para los alumnos
- Observe. What do the bands consist of?
- Explain briefly the chromatography principles.
Qué puedes esperar
<p>A series of bands is observed on the paper showing also different colours that indicate the presence of different.</p><p>Chromatography is based on the different speed of migration of the components due to their molecular interaction with solvents (mobile phase) and substrates (stationary phase).</p><p>As the paper absorbs the solvent this carries the pigments which in their turn interact with the paper moving with different speeds.</p>
Eliminación
Dispose of the filter paper and the solid mass into the solids waste container. Dispose of the solvents into the flammables waste container.
Realiza este experimento en tu clase
Este experimento forma parte del « Mobile Lab » de ATP, un laboratorio autónomo que convierte un aula normal en un laboratorio de ciencias en pleno funcionamiento, con más de 200 experimentos de física, química, biología, robótica e ingeniería. « Gali », el tutor de IA integrado en ATP Connect, guía a los alumnos paso a paso y responde a sus preguntas en la mesa de trabajo.
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